Journal: BMC Cancer
Article Title: Human non-small cell lung cancer expresses putative cancer stem cell markers and exhibits the transcriptomic profile of multipotent cells
doi: 10.1186/s12885-015-1086-3
Figure Lengend Snippet: Identification of CD166 + , CD44 + , and EpCAM + cells in cancer cell lines (A549 and H2170) and the normal bronchial/tracheal epithelial cell line (PHBEC) by flow cytometry analysis. A subpopulation of CD166 + cells was identified in the PHBEC (38.7%), A549 (72.9%), and H2170 (52.6%) cell lines. A subpopulation of CD44 + cells was identified in the PHBEC (2.2%) and A549 (61.5%) cell lines, but CD44 + cells were totally absent in the H2170 cell line. In the PHBEC, A549, and H2170 cell lines, 4.9%, 13.8%, and 33.8%, respectively, were EpCAM + .
Article Snippet: The human lung cancer cell lines A549 (lung carcinoma) and H2170 (squamous cell carcinoma) and the normal primary human bronchial/tracheal epithelium (PHBEC) cell line were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).
Techniques: Flow Cytometry